Relación entre el desempeño motor real evaluado con pruebas orientadas al proceso y el desempeño motor real evaluado con pruebas orientadas al producto: un metaanálisis / Relationship between process- and product-oriented assessments of actual motor performance: a meta-analysis

Jiménez-Díaz, J., Chaves-Castro, K. y Carpio-Rivera, E. (2023). Relación entre el desempeño motor real evaluado con pruebas orientadas al proceso y el desempeño motor real evaluado con pruebas orientadas al producto: un metaanálisis. PENSAR EN MOVIMIENTO: Revista de Ciencias del Ejercicio y la Salud, 21(1), 1-17. El desempeño motor suele ser evaluado de dos formas; la primera se enfoca en cómo se realiza la destreza, mientras que la segunda registra el resultado obtenido después de la ejecución; ambas son ampliamente usadas, sin embargo, la relación entre ellas no está clara. El objetivo del presente estudio fue determinar la relación entre los resultados de las pruebas orientadas al proceso o al producto utilizadas para medir el desempeño motor real y examinar posibles variables moderadoras por medio de la técnica meta-analítica. Para resumir los resultados, se utilizó el modelo de efectos aleatorios (REML) y se calculó el tamaño de efecto (TE) de correlación. Un total de siete estudios cumplieron con los criterios de elegibilidad, se obtuvo un total de 37 TE. Se encontró que existe una relación significativa y positiva entre el desempeño evaluado por medio de proceso y producto (TE = 0.466; IC95% = 0.391,0.541; p < .001; n = 37; Q = 78.68; p < .001; I2 = 60.17%). Como posibles variables moderadoras se analizó la edad y el tipo de destreza. Los resultados sugieren que existe una relación entre el desempeño motor real evaluado por medio de pruebas orientadas al proceso y al producto, la edad y el tipo de destreza no influyen en dicha relación.

Jiménez-Díaz, J., Chaves-Castro, K. y Carpio-Rivera, E. (2023). Relationship between process- and product-oriented assessments of actual motor performance: a meta-analysis. PENSAR EN MOVIMIENTO: Revista de Ciencias del Ejercicio y la Salud, 21(1), 1-17. Motor performance is often assessed in two different ways. The first of them focuses on how the skill is carried out, whereas the second records the product obtained after execution. While both are widely used, the relationship between them is not clear. The objective of the present study is to determine the relationship between the results of process- or product-oriented tests that are used to measure actual motor performance, and to examine possible moderating variables through the meta-analytic technique. In order to sum up the products, the random effect model (REML) was used and the effect size (TE) of the correlation was calculated. A total seven studies met the criteria for eligibility, and a total 37 TE were obtained. It was found that a significant and positive relationship exists between process- and product-evaluated performance (TE = 0.466; IC95% = 0.391, 0.541; p < .001; n = 37; Q = 78.68; p < .001; I2 = 60.17%). Age and skill type were analyzed as possible moderating variables. The results suggest that a relationship exists between process- and product-oriented assessments of actual motor performance, while age and the type of skill have no influence on that relationship.

Jiménez-Díaz, J., Chaves-Castro, K. y Carpio-Rivera, E. (2023). Relação entre o desempenho motor real, avaliado por testes orientados a processos e a produtos: uma metanálise. PENSAR EN MOVIMIENTO: Revista de Ciencias del Ejercicio y la Salud, 21(1), 1-17. O desempenho motor é geralmente avaliado de duas maneiras; a primeira se concentra em como a destreza é realizada, enquanto a segunda registra o resultado obtido após a execução; ambas são amplamente utilizadas, no entanto, a relação entre elas não é clara. O objetivo do presente estudo foi determinar a relação entre os resultados de testes orientados a processos ou a produtos usados para medir o desempenho motor real e examinar possíveis variáveis moderadoras por meio da técnica metanalítica. Para resumir os resultados, foi utilizado o modelo de efeitos aleatórios (REML) e calculado o tamanho do efeito (TDE) da correlação. Um total de sete estudos cumpriram os critérios de elegibilidade, resultando em um total de 37 TDEs. Foi encontrada uma relação significativa e positiva entre o desempenho avaliado por processo e produto (TDE = 0,466; IC95% = 0,391, 0,541; p < 0,001; n = 37; Q = 78,68; p < 0,001; I2 = 60,17%). A idade e o tipo de habilidade foram analisados como possíveis variáveis moderadoras. Os resultados sugerem que existe uma relação entre o desempenho motor real, avaliado por testes orientados a processos e a produtos, idade e tipo de habilidade não influenciam esta relação.

Porcine skeletal muscle development regulated by MicroRNA: a review / 生物工程学报

The growth and development of skeletal muscle is an important factor affecting pork production and quality, which is elaborately regulated by many genetic and nutritional factors. MicroRNA (miRNA) is a non-coding RNA with a length of about 22 nt, which binds to the 3'UTR sequence of the mRNA of the target genes, and consequently regulates its post-transcriptional expression level. In recent years, a large number of studies have shown that miRNAs are involved in various life processes such as growth and development, reproduction, and diseases. The role of miRNAs in the regulation of porcine skeletal muscle development was reviewed, with the hope to provide a reference for the genetic improvement of pigs.

Mechanisms by which exercise training benefits patients with copd mecanismos pelo qual o treinamento de exercício beneficia pacientes com dpoc running title: exercise training / Mecanismos pelos quais o treinamento de exercício beneficia pacientes com o título dpoc running: treinamento de exercício / Mecanismos por los que el entrenamiento de ejercicio beneficia a los pacientes con epoc mecanismos por los que el entrenamiento de ejercicio beneficia a los pacientes con epoc running title: exercise training

The clinical consequences of chronic obstructive pulmonary disease (COPD) include fatigue, dyspnea and progressive impairment of exercise capacity. It also produces significant systemic consequences such as nutritional depletion, physical deconditioning, systemic inflammation, and structural and functional changes in the respiratory and locomotor muscles. Regular exercise provides improvements in the health of patients with stable COPD and can relieve the symptoms, increasing the exercise capacity and improving the quality of life, while also reducing hospitalization and, to some extent, the risk of morbidity and mortality. Training with progressive exercises is associated with metabolic and neurohumoral adaptations, heart rate variability, with adaptations in the pulmonary and skeletal muscles, as well as the inflammatory, cardiovascular and respiratory systems. This review will focus on current knowledge of the mechanisms by which physical training can provide beneficial effects in COPD patients. Results: After analyzing the titles, abstracts and content, out of 500 articles found, 489 were excluded, leaving 11 articles. Studies have shown the beneficial effect of aerobic training on COPD. Conclusion: Physical training should be considered a therapeutic option in patients with COPD, regardless of being terrestrial or aquatic, as it can have beneficial effects on the systems.

As consequências clínicas da doença pulmonar obstrutiva crônica (DPOC) são: fadiga, dispnéia e comprometimento progressivo da capacidade do exercício, além disso, também produz consequências sistêmicas significativas como depleção nutricional, descondicionamento físico, inflamação sistêmica, mudanças estruturais e funcionais de músculos respiratórios e locomotores. O treinamento físico regular traz como beneficíos efeitos da melhoria da saúde em pacientes com DPOC estável e pode aliviar os sintomas, melhorar a capacidade de exercício e a qualidade de vida, reduzir a hospitalização e, em certa medida, o risco de morbi-mortalidade. Já o treinamento com exercícios progressivos está associado a adaptações metabólicas, neurohumorais, variabilidade da frequência cardíaca, inflamatórias, dos músculos pulmonares e esqueléticos, cardiovasculares e respiratórias. Esta revisão enfocará o conhecimento atual dos mecanismos pelos quais o treinamento físico pode ter efeitos benéficos em pacientes com DPOC. Resultados: Após análise dos títulos, resumos e conteúdo, dos 500 artigos encontrados, 489 foram excluídos, restando 11 artigos. Estudos têm demonstrado o efeito benéfico do treinamento aeróbico na DPOC. Conclusão: O treinamento físico deve ser considerado uma opção terapêutica em pacientes com DPOC, independente de ser terrestre ou aquático, pois pode ter efeitos benéficos nos sistemas.

Las consecuencias clínicas de la enfermedad pulmonar obstructiva crónica (EPOC) incluyen fatiga, disnea y deterioro progresivo de la capacidad de ejercicio. También produce importantes consecuencias sistémicas como el agotamiento nutricional, el desacondicionamiento físico, la inflamación sistémica y los cambios estructurales y funcionales en los músculos respiratorios y locomotores. El ejercicio regular proporciona mejoras en la salud de los pacientes con EPOC estable y puede aliviar los síntomas, aumentando la capacidad de ejercicio y mejorando la calidad de vida, al tiempo que reduce la hospitalización y, en cierta medida, el riesgo de morbilidad y mortalidad. El entrenamiento con ejercicios progresivos se asocia a adaptaciones metabólicas y neurohumorales, a la variabilidad de la frecuencia cardíaca, con adaptaciones en los músculos pulmonares y esqueléticos, así como en los sistemas inflamatorio, cardiovascular y respiratorio. Esta revisión se centrará en el conocimiento actual de los mecanismos por los que el entrenamiento físico puede proporcionar efectos beneficiosos en los pacientes con EPOC. Resultados: Tras analizar los títulos, resúmenes y contenido, de los 500 artículos encontrados se excluyeron 489, quedando 11 artículos. Los estudios han demostrado el efecto beneficioso del entrenamiento aeróbico en la EPOC. Conclusiones: El entrenamiento físico debe considerarse una opción terapéutica en pacientes con EPOC, independientemente de que sea terrestre o acuático, ya que puede tener efectos beneficiosos sobre los sistemas.

Efeitos do treinamento físico de vibração de corpo inteiro nos níveis de força de membros inferiores em idosos / Efectos del entrenamiento con ejercicios de vibración de cuerpo entero en los niveles de fuerza de miembros inferiores de adultos mayores / Effects of whole-body vibration exercises on strength levels of lower limb on elderlies

RESUMO O objetivo do presente estudo foi analisar os efeitos do exercício de vibração de corpo inteiro sobre os níveis de força nos membros inferiores de idosos. A amostra foi composta por 15 homens fisicamente ativos (idade: 62,5 ± 2,62 anos). Foram realizadas oito semanas de treinamento com plataforma vibratória, com sessões de 20 minutos de vibração utilizando frequências crescentes de 15 a 30 Hz, com exposição de 30 segundos de vibração e 30 segundos de descanso. A cada dois minutos houve um aumento de frequência de 1 Hz. Os indivíduos foram instruídos a ficar em pé, com os pés afastados na largura dos ombros, joelhos flexionados a 130°, braços estendidos, segurando firmemente a máquina. O procedimento foi realizado duas vezes por semana com intervalo de um dia. A força foi avaliada usando um dinamômetro dorsal em exercícios de extensão de joelho e extensão de coluna lombar. Os testes de força foram realizados na quarta e oitava semana após a última sessão de treinamento. Resultados: Ao final de oito semanas, foi possível observar ganhos significativos de força nos músculos envolvidos no exercício de extensão do joelho (p = 0,01) e na extensão da coluna lombar (p = 0,031). Conclusão: observou-se que o treinamento com vibrações de corpo inteiro promoveu melhora nos níveis de força nos membros inferiores dos idosos participantes do estudo.

RESUMEN El objetivo del presente estudio fue analizar los efectos del ejercicio de vibración de todo el cuerpo sobre los niveles de fuerza en los miembros inferiores de adultos mayores. La muestra estuvo formada por 15 hombres físicamente activos (edad: 62,5 ± 2,62 años). Se realizaron ocho semanas de entrenamiento con plataforma vibratoria, con sesiones de 20 minutos de vibración utilizando frecuencias crecientes de 15 a 30 Hz, con exposición de 30 segundos de vibración y 30 segundos de descanso. Cada dos minutos había un aumento de frecuencia de 1 Hz. Se indicó a los individuos que permanecieran de pie con los pies separados a la altura de los hombros, las rodillas flexionadas a 130º y los brazos extendidos sujetando firmemente los apoyamanos de la máquina. El procedimiento se realizó dos veces por semana con un intervalo de un día. La fuerza se evaluó mediante un dinamómetro dorsal en los ejercicios de extensión de rodilla y extensión de columna lumbar. Las pruebas de fuerza se realizaron en la cuarta y octava semana después de la última sesión de entrenamiento. Resultados: Al final de las ocho semanas, fue posible observar ganancias significativas en la fuerza en los músculos involucrados en el ejercicio de extensión de rodilla (p = 0,01) y en la extensión de columna lumbar (p = 0.031). Conclusión: se pudo observar que el entrenamiento con vibraciones de todo el cuerpo promovió una mejora en los niveles de fuerza en los miembros inferiores de los adultos mayores participantes del estudio.

ABSTRACT The present study was to analyze the effects of whole-body vibration exercise on strength levels in the lower limbs in elderly subjects. The sample consisted of 15 physically active individuals (62.5±2.62 years old). All volunteers were male gender. They were submitted to eight weeks of training with a vibrating platform were carried out, with sessions of 20 minutes of vibration using increasing frequencies from 15 to 30 Hz, with exposure of 30 seconds of vibration and 30 seconds of rest. Every two minutes there was an increase in the frequency of 1 Hz. The individuals were instructed to stay in the standing position with their feet shoulder-width apart, knees flexed at 130º, arms outstretched holding firmly on the machine's hand rests. The procedure was performed twice a week with an interval of one day. Strength was assessed using a dorsal dynamometer in the knee extension and lumbar spine extension exercises. Strength tests were performed in the fourth and eighth weeks after the last training session. Results: At the end of eight weeks, it was possible to observe significant gains in strength in the muscles involved in the knee extension exercise (p = 0.0001) and in the lumbar spine extension (p = 0.031) after the eight weeks of intervention. Conclusion: It was possible to observe that the whole-body vibration training promoted an improvement in the strength levels of the elderly participants in the study.

Differential expression patterns of myogenic regulatory factors in the postnatal longissimus dorsi muscle of Jeju Native Pig and Berkshire breeds along with their co-expression with Pax7

BACKGROUND: Myogenic regulatory factors (MRFs) such as MyoD, Myf6 and Myf5 play a vital role in the growth and development of muscles. Jeju Native Pig (JNP) is the top ranker in Korea amongst the indigenous livestock reared for meat purpose. Few studies covering transcript abundance of the MRFs and related to their co-expression with Pax7 in JNP have been conducted. Despite having better quality pork, JNP does not have a comparative growth rate with respect to western breeds. Therefore, the present study was designed with the objective to study the relative transcript levels of MRFs in the postnatal myogenesis of longissimus dorsi muscles in JNP and Berkshire breeds. RESULTS: Relative transcript levels were analyzed by qRT-PCR and blot expression analysis through Western blotting. Immunocytochemistry was performed to analyze their expressions at cellular levels. ToppCluster aided in the analysis of gene ontology of biological processes. The quantitative transcript levels of MyoD and Pax7 were significantly (P < 0.05) higher in Berkshire than in JNP. Myotube formation was observed under the co-expression of MyoD and Pax7. ToppCluster helped in the understanding of the linking of biological processes of the MRFs with the different signaling pathways. MyBPH had significantly (P < 0.05) high transcript levels during the chosen age groups in JNP than Berkshire. CONCLUSIONS: The current study can be helpful in understanding the genetic basis for myogenesis in postnatal stage. Moreover, it can act as stepping stone for the identification of marker genes related to body growth and meat quality in JNP.

Anthropometric and dietary assessment of patients with glycogenosis type i / Avaliação antropométrica e dietética de pacientes com glicogenose tipo i

ABSTRACT Objective: To perform anthropometric and dietary evaluation of patients with glycogenosis type Ia and Ib. Methods: This cross-sectional study is composed of a sample of 11 patients with glycogenosis divided into two subgroups according to the classification of glycogenosis (type Ia=5 and type Ib=6), aged between 4 and 20 years. The analyzed anthropometric variables were weight, height, body mass index, and measures of lean and fat body mass, which were compared with reference values. For dietary assessment, a food frequency questionnaire was used to calculate energy and macronutrients intake as well as the amount of raw cornstarch consumed. Mann-Whitney U test and Fisher's exact test were performed, considering a significance level of 5%. Results: Patients ingested raw cornstarch in the amount of 0.49 to 1.34 g/kg/dose at a frequency of six times a day, which is lower than recommended (1.75-2.50 g/kg/dose, four times a day). The amount of energy intake was, on average, 50% higher than energy requirements; however, carbohydrate intake was below the adequacy percentage in 5/11 patients. Short stature was found in 4/10 patients; obesity, in 3/11; and muscle mass deficit, in 7/11. There were no statistical differences between the subgroups. Conclusions: In patients with glycogenosis type I, there was deficit in growth and muscle mass, but no differences were found between the subgroups (Ia and Ib). Although the diet did not exceed the adequacy of carbohydrates, about 1/3 of the patients presented obesity, probably due to higher energy intake.

RESUMO Objetivo: Realizar avaliação antropométrica e dietética de pacientes com glicogenose tipos Ia e Ib. Métodos: Estudo transversal composto de uma amostra de 11 pacientes com glicogenose divididos em dois subgrupos de acordo com a classificação da glicogenose (tipo Ia=5; tipo Ib=6), com idades entre 4 e 20 anos. As variáveis antropométricas analisadas foram peso, estatura, índice de massa corporal e medidas de massa magra e gorda, que foram comparadas com valores de referência. Para avaliação dietética, foi utilizado um questionário de frequência alimentar para cálculo de ingestão de energia e macronutrientes, além da quantidade de amido cru ingerida. Realizaram-se testes U de Mann-Whitney e exato de Fisher, com nível de significância de 5%. Resultados: Os pacientes ingeriram amido cru na quantidade de 0,49 a 1,34 g/kg/dose na frequência de seis vezes ao dia, inferior à dosagem preconizada (1,75-2,50 g/kg/dose quatro vezes ao dia). A quantidade de energia consumida foi, em média, 50% a mais que as necessidades, contudo o consumo de carboidratos foi abaixo da porcentagem de adequação em 5/11 pacientes. Baixa estatura ocorreu em 4/10 pacientes, obesidade em 3/11 e déficit de massa muscular em 7/11. Não houve diferença estatística entre os subgrupos. Conclusões: Em pacientes com glicogenose tipo I, houve déficit de crescimento e de massa muscular, mas não diferença significante entre os subgrupos (Ia e Ib). Embora a dieta não tenha ultrapassado a adequação de carboidratos, 1/3 dos pacientes apresentou obesidade, provavelmente pela maior ingestão de energia.

El diafragma, el hiato y la unión gastroesofágica / The diaphragm, the hiatus and the gastroesophageal junction

RESUMEN La unión del tubo esofágico con el estómago en lo que denominamos el cardias, su tránsito y relacio nes con el hiato diafragmático, las estructuras fibromembranosas que la fijan y envuelven, la existencia de un esfínter gastroesofágico anatómico y su real morfología, así como la interacción de todos estos elementos, han sido materia de controversia por décadas y aún hoy. Este artículo actualiza la descrip ción de tales estructuras.

ABSTRACT The point where the esophagus connects to the stomach, known as the cardia, its transition and re lationship with the diaphragmatic hiatus, its fibromembranous attachments, the existence of an ana tomic gastroesophageal sphincter and its real morphology, and the interaction between all these ele ments, have been subject of debate for decades that still persist. The aim of this article is to describe the updated information of such structures.

Transcriptome profiling reveals differential expression of genes potentially involved in muscle and adipose tissue development of cattle

BACKGROUND: To identify differentially expressed genes (DEGs) between muscle and adipose in cattle, we analyzed the data from the RNA sequencing of three Angus×Qinchuan crossbred cattle. RESULTS: Searched the Gene Expression Omnibus (GEO) for a microarray dataset of Yan yellow cattle, GSE49992. After the DEGs were identified, we used STRING and Cytoscape to construct a protein­protein interaction (PPI) network, subsequently analyzing the major modules of key genes. In total, 340 DEGs were discovered, including 21 hub genes, which were mainly enriched in muscle contraction, skeletal muscle contraction, troponin complex, lipid particle, Z disc, tropomyosin binding, and actin filament binding. CONCLUSIONS: In summary, these genes can be regarded as candidate biomarkers for the regulation of muscle and adipose development.

Effect of early determinants on adolescent fat-free mass: RPS cohort of São Luís - MA

ABSTRACT OBJECTIVE: To analyze the effects of early determinants on adolescent fat-free mass. METHODS: A cohort study with 579 adolescents evaluated at birth and adolescence in a birth cohort in São Luís, Maranhão. In the proposed model, estimated by structural equation modeling, socioeconomic status (SES) at birth, maternal age, pregestational body mass index (BMI), gestational smoking, gestational weight gain, type of delivery, gestational age, sex of the newborn, length and weight at birth, adolescent socioeconomic status, "neither study/nor work" generation, adolescent physical activity level and alcohol consumption were tested as early determinants of adolescent fat-free mass (FFM). RESULTS: A higher pregestational BMI resulted in higher FFM in adolescence (Standardized Coefficient, SC = 0.152; p < 0.001). Being female implied a lower FFM in adolescence (SC = −0.633; p < 0.001). The negative effect of gender on FFM was direct (SC = −0.523; p < 0.001), but there was an indirect negative effect via physical activity level (SC = −0.085; p < 0.001). Women were less active (p < 0.001). An increase of 0.5 kg (1 Standard Deviation, SD) in birth weight led to a gain of 0.25 kg/m2 (0.106 SD) in adolescent FFM index (p = 0.034). Not studying or working had a negative effect on the adolescent's FFM (SC = −0.106; p = 0.015). Elevation of 1 SD in the adolescent's physical activity level represented an increase of 0.5 kg/m2 (0.207 SD) in FFM index (p < 0.001). CONCLUSIONS: The early determinants with the greatest effects on adolescent FFM are gender, adolescent physical activity level, pregestational BMI, birth weight and belonging to the "neither-nor" generation.

Effects of different methods of strength training on indicators of muscle fatigue during and after strength training: a systematic review

Abstract Introduction: The development of strength has shown to be beneficial to sports performance and health. However, during strength training, they also produce alterations in muscle fatigue indicators, leading to a decrease in the ability to generate strength. Despite this, there is still not enough knowledge about the levels of muscle fatigue generated by different methods of strength training and how this information can be integrated into sports planning. Review and analyze the studies existing between January 2009 and January 2019 that have used indicators of muscle fatigue established in the search terms during and after strength training as measurement variables. Evidence acquisition: The study corresponds to a systematic review of previously published studies, following the PRISMA model. Articles published between 2009 and 2019 that measured muscle fatigue indicators during and after strength training were evaluated. The electronic search was conducted through Web of Science, Scopus, Sport Discus, PubMed, and Medline. We included all articles that used a strength protocol and also measured indicators of muscle fatigue and its possible effect on physical performance. Evidence synthesis: A total of 39 articles were found, which were stratified according to the protocol used: (i) plyometric training, (ii) Bodypump® training, (iii) occlusion training, (iv) variable resistance training, (v) conventional strength training, (vi) eccentric strength training, (vii) rest times in strength training and (viii) concurrent training. Conclusion: At the end of the systematic review, it was shown that the different training methodologies for strength development generate increases in muscle fatigue indicators, and the increase generated in the different muscle fatigue indicators depends both on the methodology used and on the type of population, sex, level of training and type of sport. The most-reported indicators are [La], HR and RPE, DOM, MR variation, and ammonium.

Deficiency of Anoctamin 5/TMEM16E causes nuclear positioning defect and impairs Ca²⁺ signaling of differentiated C2C12 myotubes

Anoctamin 5 (ANO5)/TMEM16E belongs to a member of the ANO/TMEM16 family member of anion channels. However, it is a matter of debate whether ANO5 functions as a genuine plasma membrane chloride channel. It has been recognized that mutations in the ANO5 gene cause many skeletal muscle diseases such as limb girdle muscular dystrophy type 2L (LGMD2L) and Miyoshi muscular dystrophy type 3 (MMD3) in human. However, the molecular mechanisms of the skeletal myopathies caused by ANO5 defects are poorly understood. To understand the role of ANO5 in skeletal muscle development and function, we silenced the ANO5 gene in C2C12 myoblasts and evaluated whether it impairs myogenesis and myotube function. ANO5 knockdown (ANO5-KD) by shRNA resulted in clustered or aggregated nuclei at the body of myotubes without affecting differentiation or myotube formation. Nuclear positioning defect of ANO5-KD myotubes was accompanied with reduced expression of Kif5b protein, a kinesin-related motor protein that controls nuclear transport during myogenesis. ANO5-KD impaired depolarization-induced [Ca²⁺]i transient and reduced sarcoplasmic reticulum (SR) Ca²⁺ storage. ANO5-KD resulted in reduced protein expression of the dihydropyridine receptor (DHPR) and SR Ca²⁺-ATPase subtype 1. In addition, ANO5-KD compromised co-localization between DHPR and ryanodine receptor subtype 1. It is concluded that ANO5-KD causes nuclear positioning defect by reduction of Kif5b expression, and compromises Ca²⁺ signaling by downregulating the expression of DHPR and SERCA proteins.

Treinamento de força no meio aquático: uma revisão sobre os aspectos históricos, fi siológicos e metodológicos / Aquatic resistance training: a review about historical, physiological and metodological aspects

Exercitar-se na água é uma prática constatada há vários séculos, embora seja recente sua realização de forma sistemática e planejada. A literatura científi ca é ampla no estudo dos efeitos do treinamento aeróbico aquático, tendo investigado diferentes modalidades. Nas últimas décadas têm ganhado elevância os estudos que avaliam treinamentos aquáticos de caráter de força e sua melhora no sistema neuromuscular. Contudo estes trabalhos apresentam métodos de prescrição muito diferentes,dificultando sua comparação. Assim, o objetivo desta revisão é elucidar aspectos metodológicos para prescrição de exercícios de força no meio aquático. Este artigo tem caráter narrativo e para a localização e aquisição dos estudos foram consultadas as bases de dados eletrônicas SCOPUS, SCIELO e MEDLINE, realizando-se buscas de artigos publicados entre os anos 1980 e 2016, com os seguintes descritores: hidroginástica, treinamento de força aquático e corrida em piscina funda. Os resultados demonstraram que os primeiros estudos publicados mundialmente relacionados a este tema datam do início dos anos 2000. Estes seguiam métodos de treinamento que buscavam reproduzir o modelo aplicado no meio terrestre, ou seja, utilizando número de repetições e número de séries. Por outro lado, os estudos mais recentes têm proposto a utilização da velocidade máxima e o tempo de execução para enfatizar vias metabólicas específi cas (anaeróbica alática e anaeróbica lática) e promover níveis adequados de tensão mecânica (velocidade máxima) e estresse metabólico (tempo de execução). Assim, os trabalhos demonstram que o treinamento de força no meio aquático têm proporcionado resultados satisfatórios em variáveis neuromusculares e morfológicas. Ainda, a prescrição de um treinamento de força neste meio, visando a ênfase sobre vias metabólicas específi cas, utilizando o tempo de execução e a máxima velocidade, parece ser uma forma adequada de se alcançar objetivos relacionados ao treinamento de força....(AU)

Exercising in the water environment is a practice that has been observed for several centuries, although its realization in a systematic and planned manner is recent. The scientifi c literature is broad in the study of the eff ects of water-based aerobic training, having investigated diff erent modalities. In the last decades, studies that evaluate water-based resistance training and its improvement in the neuromuscular system have gained relevance. However, these studies present very diff erent prescription methods, making diffi cult to compare them. Thus, the purpose of this review is to elucidate methodological aspects for prescription of water-based resistance exercises. This manuscript is a narrative review and for location and acquisition of the studies the electronic databases SCOPUS, SCIELO and MEDLINE were searched, searching articles published between 1980 and 2016, with the following descriptors: water gymnastics, water--based resistance training and deep water running. The results showed that the fi rst published studies related to this topic date back to the early 2000s. These studies followed training methods that sought to reproduce the model applied in the land environment, that is, using number of repetitions and number of series. On the other hand, more recent studies have proposed the use of maximum velocity and time to execution to emphasize specifi c metabolic pathways (lactic and alactic anaerobic) and to promote adequate levels of mechanical stress (maximal velocity) and metabolic stress (time to execution). Thus, the studies demonstrate that water-based resistance training has provided satisfactory results in neuromuscular and morphological variables. Furthermore, prescribing strength training in this environment, aiming at emphasizing specificmetabolic pathways, using time to execution and maximum velocity, seems to be an appropriate way of achieving goals related to strength training....(AU)

Histological Changes in Biceps Muscle after Tenotomizing the Biceps Long Head in a Rat Model

BACKGROUND: Popeye deformity is common after rupture of the biceps muscle's long head tendon. Herein, we report on histological changes in biceps brachii muscles following tenotomy of the long head biceps tendon. METHODS: Twelve Sprague-Dawley rats (12-week-old) underwent tenotomy of the long head biceps tendon in the right shoulder. At postoperative weeks 4, 7, and 10, the operative shoulders were removed by detaching the biceps brachii muscle from the glenoid scapula and humerus; the opposite shoulders were removed as controls. H&E staining was performed to elucidate histological changes in myocytes. Oil-red O staining was performed to determine fatty infiltration. Myostatin antibody immunohistochemistry staining was performed as myostatin is expressed by skeletal muscle cells during myogenesis. RESULTS: H&E staining results revealed no changes in muscle cell nuclei. There were no adipocytes detected. Compared with that of the control biceps, the cross-sectional area of the long head biceps was significantly smaller (p=0.00). Statistical changes in the total extent of the 100 muscle cells were significant (p=0.00). Oil-red O staining revealed no fatty infiltration. Myostatin antibody immunohistochemical staining revealed no significant difference between the two sides. CONCLUSIONS: Muscular changes after tenotomy of the long head biceps included a decrease in the size of the individual muscle cells and in relative muscle mass. There were no changes observed in muscle cell nuclei and no fatty infiltration. Moreover, there were no changes detected by myostatin antibody immunohistochemistry assay.

Sumoylation of histone deacetylase 1 regulates MyoD signaling during myogenesis

Sumoylation, the conjugation of a small ubiquitin-like modifier (SUMO) protein to a target, has diverse cellular effects. However, the functional roles of the SUMO modification during myogenesis have not been fully elucidated. Here, we report that basal sumoylation of histone deacetylase 1 (HDAC1) enhances the deacetylation of MyoD in undifferentiated myoblasts, whereas further sumoylation of HDAC1 contributes to switching its binding partners from MyoD to Rb to induce myocyte differentiation. Differentiation in C2C12 skeletal myoblasts induced new immunoblot bands above HDAC1 that were gradually enhanced during differentiation. Using SUMO inhibitors and sumoylation assays, we showed that the upper band was caused by sumoylation of HDAC1 during differentiation. Basal deacetylase activity was not altered in the SUMO modification-resistant mutant HDAC1 K444/476R (HDAC1 2R). Either differentiation or transfection of SUMO1 increased HDAC1 activity that was attenuated in HDAC1 2R. Furthermore, HDAC1 2R failed to deacetylate MyoD. Binding of HDAC1 to MyoD was attenuated by K444/476R. Binding of HDAC1 to MyoD was gradually reduced after 2 days of differentiation. Transfection of SUMO1 induced dissociation of HDAC1 from MyoD but potentiated its binding to Rb. SUMO1 transfection further attenuated HDAC1-induced inhibition of muscle creatine kinase luciferase activity that was reversed in HDAC1 2R. HDAC1 2R failed to inhibit myogenesis and muscle gene expression. In conclusion, HDAC1 sumoylation plays a dual role in MyoD signaling: enhancement of HDAC1 deacetylation of MyoD in the basally sumoylated state of undifferentiated myoblasts and dissociation of HDAC1 from MyoD during myogenesis.

Mitochondrial dysfunction reduces the activity of KIR2.1 K⁺ channel in myoblasts via impaired oxidative phosphorylation

Myoblast fusion depends on mitochondrial integrity and intracellular Ca²⁺ signaling regulated by various ion channels. In this study, we investigated the ionic currents associated with [Ca²⁺]i regulation in normal and mitochondrial DNA-depleted (ρ0) L6 myoblasts. The ρ0 myoblasts showed impaired myotube formation. The inwardly rectifying K⁺ current (I(Kir)) was largely decreased with reduced expression of KIR2.1, whereas the voltage-operated Ca²⁺ channel and Ca²⁺-activated K⁺ channel currents were intact. Sustained inhibition of mitochondrial electron transport by antimycin A treatment (24 h) also decreased the I(Kir). The ρ0 myoblasts showed depolarized resting membrane potential and higher basal [Ca²⁺]ᵢ. Our results demonstrated the specific downregulation of I(Kir) by dysfunctional mitochondria. The resultant depolarization and altered Ca²⁺ signaling might be associated with impaired myoblast fusion in ρ0 myoblasts.

Effects of different frequencies of physical training on electron transport chain and oxidative damage in healthy mice

The present study investigated the effect of different frequencies (three and five times a week) on electron transport chain and oxidative stress after 8 weeks of run training. Methods: Eighteen male mice (CF1, 30-35g) were distributed into the following groups (n=6): untrained (UT); trained three-time per week (T3) and trained five- time per week (T5). All training sessions were at the same intensity and duration (45min/day) in a treadmill for small animals. Forty-eight hours after the last training session, the animals were killed by decapitation and quadriceps (red portion) was removed and stored at -70ºC. Succinate dehydrogenase (SDH), complexes I, II, II-III, IV and hydroperoxides were measured. Results: Training sessions for five times per week were more effective in increasing the mitochondrial respiratory chain enzyme activities (SDH, complexes I, II, II-III, IV) as well as in decreasing the formation hydroperoxides than sessions performed for three times training per week (p<0.05). Conclusion: Our findings clearly showed that a higher the frequency of training session promotes a greater activity of the electron transport chain and consequently reduces the oxidative stress in healthy animals.(AU)

Silencing MR-1 attenuates atherosclerosis in ApoE(−/−) mice induced by angiotensin II through FAK-Akt–mTOR-NF-kappaB signaling pathway

Myofibrillogenesis regulator-1 (MR-1) is a novel protein involved in cellular proliferation, migration, inflammatory reaction and signal transduction. However, little information is available on the relationship between MR-1 expression and the progression of atherosclerosis. Here we report atheroprotective effects of silencing MR-1 in a model of Ang II-accelerated atherosclerosis, characterized by suppression focal adhesion kinase (FAK) and nuclear factor kappaB (NF-κB) signaling pathway, and atherosclerotic lesion macrophage content. In this model, administration of the siRNA-MR-1 substantially attenuated Ang II-accelerated atherosclerosis with stabilization of atherosclerotic plaques and inhibited FAK, Akt, mammalian target of rapamycin (mTOR) and NF-kB activation, which was associated with suppression of inflammatory factor and atherogenic gene expression in the artery. In vitro studies demonstrated similar changes in Ang II-treated vascular smooth muscle cells (VSMCs) and macrophages: siRNA-MR-1 inhibited the expression levels of proinflammatory factor. These studies uncover crucial proinflammatory mechanisms of Ang II and highlight actions of silencing MR-1 to inhibit Ang II signaling, which is atheroprotective.

Muscle dysmorphia and use of ergogenics substances. A systematic review / Dismorfia muscular y uso de sustancias ergogénicas. Una revisión sistemática

Abstract The use of ergogenic substances (UES) is not restricted to achieving a better athletic performance, but also it is a behaviour for body changing through muscle development; however, little is known about the relationship between muscle dysmorphia (MD) and UES. Therefore, it was conducted a systematic review of those empirical papers that have studied this relationship over the last decade (2004-2014). First it is highlighted that of the 22 articles analysed, only 13 explicitly aimed this interest. Besides, although the documented data outlined some relevant aspects such as the existence of a high co-occurrence (60-90%) between MD and UES. In general, the evidence is still incipient and uncertain, mainly because of the large disparity between the methodologies of the studies, particularly in terms of indicators, parameters and measures utilised to assess UES within the context of MD.

Resumen El uso de sustancias ergogénicas (USE) no se restringe a la consecución de un mayor desempeño atlético, actualmente también es una conducta de cambio corporal, vía el desarrollo muscular; no obstante, poco se sabe de la relación entre dismorfia muscular (DM) y USE. Por tanto se realizó una revisión sistemática de los estudios empíricos que, durante la última década (2004-2014), la han examinado. De entrada, destaca el hecho de que, de los 22 artículos analizados, solo en 13 se explicita este interés. Además, aunque los datos documentados delinean algunas vertientes relevantes, como la existencia de una alta concomitancia (60-90%) de DM y USE, en general las evidencias son aún incipientes e inciertas, principalmente debido a la gran disparidad metodológica entre estudios y, particularmente, en cuanto a los indicadores, los parámetros y las medidas que, en el contexto de la DM, se han venido empleando para evaluar USE.

The expression of gene ANKRD1 correlates with hypoxia status in muscle biopsies of treatment-näive adult dermatomyositis / A expressão do gene ankrd1 correlaciona-se com o status de biópsias musculares em adultos portadores de dermatomiosite sem exposição prévia a tratamento

OBJECTIVES. The ANKRD1 gene codes for the ankyrin repeat domain containing protein 1 and has an important role in myogenesis and possibly also in angiogenesis. Microvasculopathy is a cornerstone and an early pathological marker of change in dermatomyositis, leading to hypoxia and muscle perifascicular atrophy. These alterations could upregulate genes involved in myogenesis and angiogenesis such as ANKRD1. Therefore, we analyzed ANKRD1 expression in muscle biopsies of dermatomyositis and correlated with other hypoxia parameters and with histological changes. METHODS. Total RNA was extracted from frozen muscle biopsies samples of 29 dermatomyositis patients. A control group consisted of 20 muscle biopsies from adult patients with non-inflammatory myopathy diseases. The gene coding for hypoxia-inducible factor 1, alpha subunit (HIF1A), was analyzed to estimate the degree of hypoxia. ANKRD1 and HIF1A transcript expression levels were determined by quantitative real time PCR. RESULTS. Significantly higher ANKRD1 and HIF1A expression levels were observed in dermatomyositis relative to the control group (P<0.001, both genes). In addition, ANKRD1 and HIF1A were coexpressed (r=0.703, P=0.001) and their expression levels correlated positively to perifascicular atrophy (r=0.420, P=0.023 and r=0.404, P=0.030, respectively). CONCLUSIONS. Our results demonstrate ANKRD1 overexpression in dermatomyositis correlated to HIF1A expression and perifascicular atrophy. ANKRD1 involvement in myogenesis and angiogenesis mechanisms indicates that further investigation is worthwhile.

OBJETIVOS: ANKRD1 codifica "ankyrin repeat domain containing protein 1" e tem um papel importante na miogênese e possivelmente também na angiogênese. Microvasculopatia é considerada como um ponto central e uma alteração patológica precoce na dermatomiosite (DM), levando à hipóxia e à atrofia perifascicular muscular. Estas alterações poderiam estimular genes envolvidos na miogênese e angiogênese como ANKRD1. Portanto, analisamos a expressão de ANKRD1 em biópsias musculares de DM e correlacionamos com outros parâmetros de hipóxia e alterações histológicas. MÉTODOS: O RNA total foi extraído de biópsias de músculos congelados de 29 pacientes com DM. Como grupo controle, foram usadas 20 biópsias de músculo de pacientes adultos com miopatia não-inflamatória. O gene que codifica a subunidade alfa do fator 1 induzido por hipóxia (HIF1A) foi analisado para estimar o grau de hipóxia. Os níveis de expressão dos transcritos ANKRD1 e HIF1A foram determinados por PCR quantitativa em tempo real. RESULTADOS: Níveis aumentados de expressões de ANKRD1 e HIF1A foram observados em DM quando comparados ao grupo controle (P<0,001, ambos os genes). Além disso, ANKRD1 e HIF1A apresentaram coexpressão (r=0,703, P=0,001) e seus níveis de expressão correlacionaram-se também positivamente com atrofia perifascicular (r=0,420, P=0,023 e r=0,404, P=0,030, respectivamente). CONCLUSÕES: Nossos resultados demonstraram aumento de expressão de ANKRD1 na DM, que correlacionou com a expressão de HIF1A e atrofia perifascicular. Investigações adicionais do envolvimento de ANKRD1 no mecanismo de miogênese e angiogênese devem ser realizadas.

Impacto en las características morfológicas del músculo esquelético de los peces criados en granja / Impact in the morphological characteristics of the skeletal muscle of fish farm species

Introducción: El pescado es una fuente completa de proteínas de fácil digestión. Su aceite, vitaminas y minerales proporcionan un funcionamiento normal a todos los sistemas básicos del organismo. Estudiar las características del crecimiento postnatal del músculo esquelético del pez permite formular propuestas basadas en evidencias para la producción de carne de pescado de buena calidad. Material y Métodos: Estudio prospectivo de más de 300 especies de peces bajo principios análogos: edad joven de 20 a 500 días, de 860 días y con 2 a 3 años bajo crianza con tecnología de granja. El complejo metodológico incluyó morfometría, disección anatómica, métodos de preparación histológica, microscopio electrónico (Quanta 200 3D) y procesador estadístico. Resultados: Las condiciones tecnológicas y la conducta alimentaria garantizan el crecimiento del pez tipo pequeño (fingerlings). Según los registros del estudio, el incremento del peso del cuerpo de los peces carpa entre 15 y 135 días, fitoplankton para carpas plateadas sobre 190 y 301 veces, fitovorous para carpa herbívora, 128 a 302 veces. Discusión: El crecimiento vigoroso hiperplásico en el período inicial de la ontogénesis influye en el crecimiento posterior de los animales adultos. La dinámica estacional de las características morfométricas de las fibras musculares está influida por la intensidad de los procesos metabólicos del organismo.

Introduction: Fish as a food product is a source of complete, easily digested proteins, fish oil, vitamins, minerals which provide normal operation of all basic systems in the organism. Studying the general and specific characteristics of postnatal growth of fish skeletal muscles allow us to formulate evidence-based approach to the production of good quality fish. Methods: Prospective study of more than 300 species under analogue principles: : juveniles aged from 20 till 500 days, species aged 860 days at two- and three years farming technology. Complex methodological approach including morphometrics, anatomic dissection, histological set up methods with electronic scanning microscopy (Quanta 200 3D) was used. Statistical data processing was performed. Results: Technological conditions and feeding behaviour guarantee the intensity of fingerlings growth. According to our records carp increases body weight up to from 50 (at 3 year technology) to 411 times (at 2 year technology) between 15 and 135 days; phytoplankton silver carp up to 190-301 times, phytovorous grass carp up to 128-302 times respectively. Discussion: Vigorous hyperplastic growth of carps at early stages of postnatal ontogenesis influences on further growth of mature animals. Seasonal dynamics of morphometric characteristics of muscular fibers is determined by intensity of metabolic processes proceeding in organism.